Enzyme immobilization

Enzyme immobilization is a method in which an enzyme of interest is entrapped in a gel matrix. That can be use in other experiments.

Alginate(alginic acid) along with sodium salt which is sodium alginate is soluble in water, but when this alginate is present with a divalent cation, like Ca2++, it becomes water insoluble.

Materials

1. Sodium alginate solution.

2. Enzyme of our interest.

3. calcium chloride solution.

4. Syringe.

Procedure

1. Specific amount of enzyme is mixed in sodium alginate solution.

2. Using a syringe this mixture is drop wise poured in calcium chloride solution.

3. Beads formation occurs.

Result
White or transparent gel beads form.

When the mixture is poured in calcium chloride solution, the sodium ion of the alginate is substituted by the calcium ion and sodium alginate is converted to calcium alginate, which is insoluble to water that's why forms bead. The enzyme is entrapped inside the gel beads.


Hope this post will help you 
                                                (~Bio blog)



Other posts

http://bioblogsworld.blogspot.com/2022/07/gram-staining-method.html

http://bioblogsworld.blogspot.com/2022/07/negative-staining-method.html


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