Gram staining method
In this post i am going to describe gram staining method in brief. Introduction Gram staining procedure is a differential staining method used to differentiate between gram positive and gram negative bacteria. First used by Hans Christian Gram. Gram positive bacteria stains violet or dark blue. Gram negative bacteria stains red. Materials Crystal violet; used as a primary stain Safranin; used as a counter stain Grams iodine Glass slide Distilled water Decolorizer (ehtanol) Inoculating loop Procedure 1. Take the slide and wash it,dry it. 2. Take a drop on the slide. 3. Using inoculating loop take the bacterial sample and mix it in the water drop. 4. Make a smear of the water drop. 5. Air dry the smear. 6. Heat fix the smear. 7. Add crystal violet on the smear for 45sec to 1min. 8. Then add grams iodine(you can also wash the crystal violet with distilled water before adding iodine) and wait for 30 sec to 45 sec. 9.wash with decolorizer. 10. Add safranin. Wait for 45 sec to 1 min. 11. was...