pbr322

pbr322 is a plasmid vector.

P stands for plasmid, B is for bolivar ar R is for Rodriguez.
Contains an origin of replication, an ampicillin resistance and a tetracycline resistance gene.
Contains 40 restriction sites, 11 of then are in tet resistance gene and 6 of them are in amp resistance gene.
Others are present throughout the plasmid.

Source of these genes are-
Ori site - pMB1 plasmid
amp resistance gene - rsf2124
Tet resistance gene - psc101



EcoR1 restriction site is denoted as 0 .

HindIII restriction site is present on the promoter region of tet gene. Treated with this restriction enzyme, causes inactivation of tetracycline resistance.
Rop gene is present which controls the copy number.
hope this post will help (~Bio blog)


Other posts

http://bioblogsworld.blogspot.com/2022/07/enzyme-immobilization.html

http://bioblogsworld.blogspot.com/2022/07/malolactic-fermentation.html
https://bioblogsworld.blogspot.com/2022/12/corynebacterium-diphtheriae.html

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